Question

Why doesn't synthesis of free glucose residues take place in the cytosol

Answer 1

Answer:

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Explanation:

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Answer 2

The first reaction is catalyzed by pyruvate carboxylase and the second by phosphoenolpyruvate carboxykinase. The sum of these reactions is:

Pyruvate carboxylase is of special interest because of its structural, catalytic, and allosteric properties. The N-terminal 300 to 350 amino acids form an ATP-grasp domain (Figure 16.25), which is a widely used ATP-activating domain to be discussed in more detail when we investigate nucleotide biosynthesis (Section 25.1.1). The C-terminal 80 amino acids constitute a biotin-binding domain (Figure 16.26) that we will see again in fatty acid synthesis (Section 22.4.1). Biotin is a covalently attached prosthetic group, which serves as a carrier of activated CO2. The carboxylate group of biotin is linked to the ε-amino group of a specific lysine residue by an amide bond (Figure 16.27). Note that biotin is attached to pyruvate carboxylase by a long, flexible chain.