Why don t restriction enzymes digest bacteria dna?
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Restriction enzymes are part of the restriction-modification system; bacteria and Archea developed thus system as a defense against viruses. Bacterial methylases protect the host DNA by methylation in the sequences the restriction enzymes recognize, and the restriction enzymes fail to cleave methylated DNA. The foreign DNA (viral) on the other hand, gets chopped up so the infection is avoided.
These enzymes became a fundamental tool of molecular biology and allowed molecular cloning to develop. Because the sequence of the recognition sites was known, physical maps of DNA could be made by double or triple cutting, etc. They are still widely used in most molecular biology labs, there are thousands of them.
These enzymes became a fundamental tool of molecular biology and allowed molecular cloning to develop. Because the sequence of the recognition sites was known, physical maps of DNA could be made by double or triple cutting, etc. They are still widely used in most molecular biology labs, there are thousands of them.
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