Question

why enzyme activity does not exceed its maximum velocity after further rise substrate concentration? ​

Answer 1

Answer:

It has been shown experimentally that if the amount of the enzyme is kept constant and the substrate concentration is then gradually increased, the reaction velocity will increase until it reaches a maximum. After this point, increases in substrate concentration will not increase the velocity.

Answer 2

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Here is your answer

Question => why enzyme activity does not exceed its maximum velocity after further rise substrate concentration?

Answer => Substrate concentration: Increasing substrate concentration also increases the rate of reaction to a certain point. Once all of the enzymes have bound, any substrate increase will have no effect on the rate of reaction, as the available enzymes will be saturated and working at their maximum rate.