Why fluorescent sampled can not be studied using raman spectroscopy?
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Raman spectra of solutions with very low concentrations of amino acids 1mM-6 mM. However, I can not see their peaks because they are masked by the fluorescence of the solutions, which also prevents me from using long exposure times. I have no idea what is the source of this fluorescence because my buffer is 25 mM in Tris-HCl and 150 mM in NaCl (pH=7.2). Also, I always wash my cuvettes with soap and nitric acid before using them. Does anyone have any tips on how to decrease the fluorescence? By the way, I am using a 514 nm laser source.
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