Why gel electrophoresis is said to be very important technique in recombinant dna technology?
Answers
Gel electrophoresis is one of the techniques widely used in the field of recombinant DNA technology for the separation of proteins. Among the varieties SDS PAGE is most popular method which separates the proteins based on molecular weights and charge/mass ratio.The proteins with more more mol.weight travel slower when compared to proteins with low mol.weight.Sodium Do-decyl Sulphate is anionic exchanger which imparts a partial negative charge to the proteins.The process includes various kinds of buffers and chemicals, loading dyes.The proteins are initially subjected to lysis to bring them from tertiary structure to primary.When electricity is applied due to variations in the ph of tank buffer and running buffer the proteins migrate towards their opposite charge and get separated based on their molecular weights.This is just a brief description of SDS PAGE.The more deeper we go the more mechanism is involved