Biology, asked by razk3120, 1 year ago

Why half protein is staying in gel itself during western blot transfer?

Answers

Answered by surender824
0
The thing is the MWM it seems to be tranfer but there´s no protein on the membrane or gel.

i saw online a few photos of the ponceau S stain and the bands are pretty obvious.

maybe the proteins are passing through, but is strange because there are a 100 kDa proteins in my sample. i don´t know if is the setting or what. the buffer is the Towbin buffer, is the most common, 

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