Why heated phosphate buffer is used to dissolve gram powder for extraction of urease?
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Milky substances in the extract were removed by heat treatment. Urease in the clear yellow supernatant was precipitated by adjusting the pH of the solution to 5.4 with citric acid. The acid precipitated urease was neutralized by dissolving in 0.015 M phosphate buffer, pH 8.5 (final pH 6.8 to 7.0) and then lyophilized.
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Urea Agar Base with the addition of Urea is recommended for the detection of urease production, particularly by members of the genus Proteus . Suspend 24.01 grams in 950 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes.
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