Question

Why high temperature required for denaturation in pcr?

Answer 1

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This bacteria normally lives in hot springs so can tolerate temperatures above 80⁰C. The bacteria's DNA polymerase is very stable at high temperatures, which means it can withstand the temperatures needed to break the strands of DNA apart in thedenaturing stage of PCR.
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Answer 2

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The three step PCR processes is initiated. during the first step of PCR, called denaturation, the tube containing the sample DNA is heated to more than 90 degree Celsius, which separates the double standed DNA into two separate strands.