Question

Why iodine spread on plate to detect amylase producing bactera?

Answer 1

Amylases are amongst most widely used enzymes in industries such as food, fermentation, starch processing, textile and paper. In the present investigation, bacteria were isolated from potato dump sites of different regions of Madhya Pradesh, screened for the production of amylase and their optimum growth conditions were determined. A total of 25 bacterial colonies were isolated from collected soil samples. Five bacterial isolates, displayed zones of clearance in starch hydrolysis test. The isolate displaying maximum amylase activity on quantitation was selected. Characteristic feature of the strain indicates that it belongs to the genus Bacillus and will be later used for further characterization. Maximum yield of amylase was obtained after 48hrs of incubation. The optimum pH for enzyme activity was found to be at pH 7.0 and the optimum temperature for the activity was found to be at 40ºC.

Answer 2

Explanation:

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Answer:

The correct answer is deep blue colour.