Question

Why is charging of trna necessary during translation process?

Answer 1

Charging of the tRNA in the sense of loading an amino acid onto the tRNA is therefore essential for protein synthesis as otherwise ribosomes could not convert the genetic code used in mRNAs into the chain of amino-acids as found in protein

Answer 2

tRNA charging: In the presence of ATP, amino acids are activated and connected to their cognate tRNA. The process is known as tRNA charging or amino-acylation. When two charged tRNAs are brought near enough together, the creation of a peptide bond between the respective amino acids is energetically favoured. The inclusion of a catalyst would speed up the creation of peptide bonds.