Question

Why is double-strength broth used in some tubes of the mpn series?

Answer 1

hi
 Double-strength broth is use when diluTng large amount of samples (10ml) for the test Single-strength broth can be used for sample such as 1ml 0.1ml because they don not dilute the broth very much

Answer 2

Double-strength broth used in some tubes of the mpn series because in single strength 10 ml of sample is mixed with 10 ml of broth which will get diluted due to which growth will not be accurate but in double strength, due to high concentration all the cell will get proper nutrient and thus,give positive and accurate result.