why is it essential to boil the leaf in water first and then in ethanol?
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the boiling procedure kills a leaf, disrupts the cell membranes and softens the cuticle and cell walls. This makes it possible to extract the chlorophyll with hot ethanol and also allows the iodine solution to penetrate the cells and react with any starch present..
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Hi hope this helps you...
This process is mainly for testing the presence of starch on the leaf.Boiling the leaf in water kills the leaf, disrupts the cell membranes and softens the cuticle and cell walls. This makes it possible to extract the chlorophyll with hot ethanol and also allows the iodine solution to penetrate the cells and react with any starch present.
This process is mainly for testing the presence of starch on the leaf.Boiling the leaf in water kills the leaf, disrupts the cell membranes and softens the cuticle and cell walls. This makes it possible to extract the chlorophyll with hot ethanol and also allows the iodine solution to penetrate the cells and react with any starch present.
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