Question

Why is it imp to remove affinity tag from recombinant protein for vaccine generation?

Answer 1

Answer:

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10-Jan-2012 · In contrast to affinity purification, the genetically engineered HaloTag® (Promega) has been designed to enhance expression and solubility of recombinant proteins in bacterial hosts, as well as to provide an efficient means of protein purification coupled with tag removal by TEV protease‐mediated proteolytic cleavage

Answer 2

Answer:

I have purified my protein (6kD) from pET28a+. I want to proceed with antibody production against this protein.