Question

why is leishmans stain diluted after two minutes and not earlier ? give reason​

Answer 1

This will stain the blood cells. ⇒ Rinse the slides thoroughly with Phosphate buffer solution up to 2 minutes or until it acquires a purple-pinkish tinge. ⇒ Air dry the slides in a tilted position so that the water easily remove out of the slides.

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The color of Cytoplasm by Leishman Stain.

Erythrocytes Pink

Basophils Blue