Question

why is molar mass an important parameter​

Answer 1

Answer:

The molecular mass of an intact protein provides a frame within which the final structure must fit. The molecular mass information is an important analytical parameter required in many situations. Top-down proteomics relies on the molecular mass of intact proteins. This information is also needed to verify the correctness of the translated sequence of a protein and to identify point mutations, to find the extent of PTMs, and to determine the number of cysteine residues and disulfide bonds. ESI and MALDI are the methods of choice for determining the molecular mass of intact proteins. The ESI of high-mass biopolymers produces several multiply charged ions, the deconvolution of which gives the accurate molecular mass of the target protein. MALDI produces a simpler spectrum that contains mainly singly protonated ions.

Explanation:

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