Question

why is not a detegent like sodium dodecyl sulfate alone sufficient to lyse plant cells ? (explain in 200 word.​

Answer 1

Answer:

Detergents are a class of molecules whose unique properties enable manipulation (disruption or formation) of hydrophobic–hydrophilic interactions among molecules in biological samples. In biological research, detergents are used to lyse cells (release soluble proteins), solubilize membrane proteins and lipids, control protein crystallization, prevent nonspecific binding in affinity purification and immunoassay procedures, and are used as additives in electrophoresis.

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Answer 2

Answer:

Detergents can be denaturing or non-denaturing with respect to protein structure. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking protein–protein interactions.

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