Question

Why is polyamide stationary phase used in separation of compounds in HPLC?​

Answer 1

Answer:

Due to the medium polarity of polyamide the stationary phase (polyamide swollen with eluent) can be used with mobile phase that is more polar or less polar. ... Thus, structural isomers can be well separated on polyamide.

Answer 2

Polyamide stationary phase used in separation of compounds in HPLC due to the medium polarity of polyamide .

Explanation:

• Because of polyamide's medium polarity, the stationary phase (polyamide swollen with eluent) can be utilised with more or less polar mobile phases.
• Silica is the most often used polar and acidic support in adsorption chromatography.
• The surface silanol groups on this support absorb polar chemicals and function especially well with basic molecules.
• Alumina is the most often used polar and basic adsorbent in adsorption chromatography.

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