why is staining required?
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The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.
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Cell staining is important in the diagnosis of microorganisms because bacteria can be identified by the color differentiation of stains (dyes). Microscopic examination of stained cell samples allows examination of the size, shape, and arrangement of organelles, as well as external appendages such as the whip-like flagella, which are the cell's organs of motion. When sample cells are stained to show their chemical composition it is called differential staining.
The main reason you stain a specimen before putting it under the microscope is to get a better look at it, but staining does much more than simply highlight the outlines of cells. Some stains can penetrate cell walls and highlight cell components, and this can help scientists visualize metabolic processes. Stains also help distinguish between live cells and dead ones. Moreover, staining allows scientists count the number of cells of a particular type within a certain biomass. Twenty or more different types of stains exist, and each one has its purpose.
The main reason you stain a specimen before putting it under the microscope is to get a better look at it, but staining does much more than simply highlight the outlines of cells. Some stains can penetrate cell walls and highlight cell components, and this can help scientists visualize metabolic processes. Stains also help distinguish between live cells and dead ones. Moreover, staining allows scientists count the number of cells of a particular type within a certain biomass. Twenty or more different types of stains exist, and each one has its purpose.
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