Question

why is the use of stain is necessary​

Answer 1

Answer:

The most basic reason is that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope

Explanation:

Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.

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Answer 2

the use of stain is necessary so that it can then be stain, otherwise, your bacterial sample would literally wash off when you pour water.