Why it is preferred that vector should have only single recognition site??
pls answer it fast..
Answers
Selecting the right vector for your molecular biology experiment is fundamental to ensure the experiment’s success. There are many elements to consider when choosing which vector backbone to use. To make it easier, we have compiled a list of some of the key factors to keep in mind when choosing a vector:
This factor is relevant for both cloning and expression vectors. The only aspect to consider here is whether you are cloning a large or small DNA fragment. Most general plasmids (i.e. pUC) can cope with inserts up to 15 kb. Anything bigger can complicate the replication and cause problems with stability. However, if you have a large DNA insert, there are special types of vectors suitable for such applications, which have a different origin of replication. As a general remark, origins of bigger vectors have a lower copy number than those of smaller vectors. Keep in mind that the bigger the vector is, the lower the transfection or transformation efficiency and its stability. Nevertheless, vectors can usually get up to 52 kb.