Why linearity is lost in uv spectroscopy at higher wavelength?
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Explanation:
Lambert Beer law at high concentrations cannot give good correlations because when the absorbance is higher than 1, it is absorbed all light. Under these conditions the absornabance is not coorrect (see definition of absorbance).
At low concentrations, lower than 0.04 the measured has to much error, this leads to important precision of the absorbance measurement.
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