Chemistry, asked by tesla3300, 1 year ago

Why mgcl2 cacl2 in competent cell preparation kept in ice?

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Answered by Ashwini26
0

The addition of mgcl2 cacl2 to a cell suspension promotes the binding of plasmid DNA to lipopolysaccharides (LPS).

It also acts as a preserving agent as the prepared competent cells need to be stored at 80 °C for a longer period without losing their viability. .....

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