Why must very thin samples be used under a light microscope?
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Thin sections of specimen are needed for transmission electron microscopy as the electrons have to pass through the specimen for the image to be produced
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Explanation:
Through a light microscope, the sample has to be transparent. If the microscope has any kind of objective (as for mainly all optical microscopes) the depth of focus is limited, thus allowing only for a limited thickness inside the sample to be imagined sharp. All layers that are out of focus contribute to the overall noise of the image thus degrading image quality. These are the two main reasons when regarding optical microscopes, although sample requirements might differ e.g. for TEMs or AFMs.
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