Question

Why must young cultures be used when doing a Gam stain?​

Answer 1

Answer:

• Why must young cultures be used when doing a Gram stain Young cultures must be used so the crystal violet can stick to the cell walls of Gram positive bacteria. The cell walls break down in old cultures and the staining process is not accurate.

Answer 2

ᴄᴏʀʀᴇᴄᴛ ǫᴜᴇsᴛɪᴏɴ :-

ᴡʜʏ ᴍᴜsᴛ ʏᴏᴜɴɢ ᴄᴜʟᴛᴜʀᴇs ʙᴇ ᴜsᴇᴅ ᴡʜᴇɴ ᴅᴏɪɴɢ ᴀ ɢᴀᴍ sᴛᴀɪɴ?

ᴄᴏʀʀᴇᴄᴛ ᴀɴsᴡᴇʀ :-

ᴄᴜʟᴛᴜʀᴇs ᴍᴜsᴛ ʙᴇ ᴜsᴇᴅ sᴏ ᴛʜᴇ ᴄʀʏsᴛᴀʟ ᴠɪᴏʟᴇᴛ ᴄᴀɴ sᴛɪᴄᴋ ᴛᴏ ᴛʜᴇ ᴄᴇʟʟ ᴡᴀʟʟs ᴏғ ɢʀᴀᴍ ᴘᴏsɪᴛɪᴠᴇ ʙᴀᴄᴛᴇʀɪᴀ. ᴛʜᴇ ᴄᴇʟʟ ᴡᴀʟʟs ʙʀᴇᴀᴋ ᴅᴏᴡɴ ɪɴ ᴏʟᴅ ᴄᴜʟᴛᴜʀᴇs ᴀɴᴅ ᴛʜᴇ sᴛᴀɪɴɪɴɢ ᴘʀᴏᴄᴇss ɪs ɴᴏᴛ ᴀᴄᴄᴜʀᴀᴛᴇ.

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