Why nahco3 sodium bicarbonate use in RBC lysis buffer?
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I was using Potassium Bicarbonate to make my RBC lysis buffer but now that salt is no more available in market. Is there a substitute for it?
It was made along with Ammonium Chloride and Disodium EDTA + Potassium Bicarbonate.
From this Potassium Bicarbonate is no more sold in market by any brands. SIGMA was the last option but even they have removed it from their catalog.
Any thoughts? I was using it from many years.
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