Why phage vectors are preffered over plasmid vectors for genomic library preparation?
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set of techniques adopted for gene cloning for a particular purpose is said to be a gene cloning strategy. There are several gene cloning strategies in recombinant technology. DNA fragments are generated by cutting the DNA with a specific restriction endonuclease. These fragments are ligated into a vector molecules and the collection of recombinant molecules is transferred into host cells, one molecule in each cell. The total number of all DNA molecules makes up the library. Two main types of libraries can be used to isolate specific DNAs: genomic and cDNA libraries.
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