Question

Why polymerase enzymes are kept in -20 degree celcius

Answer 1

Usually you will use a heat stable Polymerase for PCR (e.g. Taq Poylmerase, "Taq's optimum temperature for activity is 75–80°C, with a half-life of greater than 2 hours at 92.5°C, 40 minutes at 95°C and 9 minutes at 97.5°C" . Other, non heat stable, polymerases will of course denature.

You can test the functionality of your polymerase by running PCR with different temperatures, and check if you get you desired product.

Answer 2

Answer:they are more fast in catalysing the reactions..

Explanation:

So they have to be put to that temperature for long storage ,When waiting for the substrate