why pond water is used to determine that oxygen is released during photosynthesis?
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When plant leaves absorb carbon dioxide from the air they combine it with waterfrom the roots to make sugars for food but they usually have some left over oxygen so this is released out of the leaf. We can collect this oxygen to measure photosynthesis.
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Photosynthesis is a physiological process of green plants to manufacture their carbohydrate food. It is a complex chemical process occur in the chloroplasts of mesophyll tissue of green plants. During photosynthesis process green plants utilizes energy from solar radiation, Co2 from atmosphere, water and minerals from obtained from soil to prepare their carbohydrate food and in turn evolve oxygen.
Take a beaker and fill 3/4ths of it with pond water. Take some freshly cut Hydrilla twigs and insert them in the nozzle from below the stem of a glass funnel. Keep this funnel in an inverted position at the bottom of the beaker. A beehive arrangement is used in between the base of the funnel and bottom of the beaker. Now a test tube is filled with pond water and is inverted over the stem of the funnel. While making this arrangement one has to see the level of water in the beaker should be above the level of stem of the inverted funnel. The whole arrangement is kept on a flat surface under bright sunlight for some time.
Air bubbles start moving from the cut end of the Hydrilla twigs and get collected at the upper end of the test tube. After some time we find some gas get collected at the top of the inverted test tube by downward displacement of water. To test the nature of the gas collected, the test tube is removed quickly from the stem of the funnel and the mouth is closed with the thumb. Now a glowing splinter is introduced into the test tube by removing the thumb from the mouth of the test tube. The glowing splinter immediately burst into a flame indicating that the gas collected in the test tube is oxygen.
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Take a beaker and fill 3/4ths of it with pond water. Take some freshly cut Hydrilla twigs and insert them in the nozzle from below the stem of a glass funnel. Keep this funnel in an inverted position at the bottom of the beaker. A beehive arrangement is used in between the base of the funnel and bottom of the beaker. Now a test tube is filled with pond water and is inverted over the stem of the funnel. While making this arrangement one has to see the level of water in the beaker should be above the level of stem of the inverted funnel. The whole arrangement is kept on a flat surface under bright sunlight for some time.
Air bubbles start moving from the cut end of the Hydrilla twigs and get collected at the upper end of the test tube. After some time we find some gas get collected at the top of the inverted test tube by downward displacement of water. To test the nature of the gas collected, the test tube is removed quickly from the stem of the funnel and the mouth is closed with the thumb. Now a glowing splinter is introduced into the test tube by removing the thumb from the mouth of the test tube. The glowing splinter immediately burst into a flame indicating that the gas collected in the test tube is oxygen.
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