Why rbc is not visible after putting wbc diluting fluid?
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Explanation:
RBC diluting fluid is isotonic with blood, hence hemolysis does not take place. Normal Saline also can be used. But it causes slight creation of red blood cells and allows rouleaux formation. ... The number of cells in undiluted blood are calculated and reported as the number of Red cells per cu mm (MI) of whole blood.
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Explanation:
Glacial acetic acid lyses the red cells. Gentian violet slightly stains the nuclei of the leucocytes. The blood specimen is diluted 1:20 in a WBC pipette with the diluting fluid and the cells are counted under low power of the microscope by using a counting chamber.
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