Question

Why recombinant factor 8 gene of eukaryotes could not be expressed in prokaryotes while insulin can?

Answer 1

many proteins such as human insulin, human growth hormone and a wide range of pharmaceuticals are mass-produced from genetically engineered bacteria and fungi. shows an example in which the genetic engineering resulted in a drug that happened to give a strikingly different color to the bacterial colony when streaked onto a petri plate.

Expression of human insulin in E. coli. The two chains of insulin are made separately as fusion proteins with β-galactosidase. They are processed chemically and then mixed, and active insulin forms.