Chemistry, asked by AdhyayanDubey1326, 10 months ago

Why salt gradient is used in ion exchange chromatography?

Answers

Answered by anoopnegi311

A salt gradient is used to elute seperated proteins. At low salt concentration , proteins having few charged groups are eluted and at heigher salt concentration proteins with several charged groups are eluted . Unwanted proteins and impurities are removed by washing the coloumn.

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