Chemistry, asked by SaI20065, 8 months ago

why should electrophoresis be done in solution of low salt concentration ?​

Answers

Answered by kalavathibathina1717
3

Answer:

Electrophoresis,larger molecules are resolved better using a low concertration gel while smaller molecules separate better at high concentration gel.High concentration gel howere requires longer run times

Answered by mohdmunshi87
1

Answer:

please follow me and mark me as brilliant. I beg in front of you.

Explanation:

High Salt Concentrations in Samples

High salt concentrations result in increased conductivity that affects protein migration, and can result in gel artifacts in adjacent lanes containing samples with normal salt concentrations. Perform dialysis, or precipitate and resuspend samples in lower-salt buffer prior to electrophoresis.

Similar questions