why should electrophoresis be done in solution of low salt concentration ?
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Answer:
Electrophoresis,larger molecules are resolved better using a low concertration gel while smaller molecules separate better at high concentration gel.High concentration gel howere requires longer run times
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Explanation:
High Salt Concentrations in Samples
High salt concentrations result in increased conductivity that affects protein migration, and can result in gel artifacts in adjacent lanes containing samples with normal salt concentrations. Perform dialysis, or precipitate and resuspend samples in lower-salt buffer prior to electrophoresis.
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