Why should we use normal salin for bacterial dilution?
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Normal" saline used in Microbiology has the same osmolarity as most intra cellular fluid. So when you add normal saline to suspend microorganisms in a specimen or culture, these microorganisms will not rupture due to either intracellular fluid coming out of the saline going in due to osmosis through the cell membrane.
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HERE IS YOUR ANSWER
___======_____==_____
Normal" saline used in Microbiology has the same osmolarity as most intra cellular fluid. So when you add normal saline to suspend microorganisms in a specimen or culture, these microorganisms will not rupture due to either intracellular fluid coming out of the saline going in due to osmosis through the cell membrane.
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