Question

Why sodium lauryl sulfate inhibits non coliform bacteria?

Answer 1

Lauryl Tryptose media contains sodium lauryl sulfate, which inhibiting gram-positive microorganisms, serves as a selective agent for coliforms. The addition of lactose to the medium allows for detection of rapid lactose fermentation by coliforms. Coliforms grown in Lauryl Tryptose Broth ferment lactose and produce gas. Other bacteria are either inhibited or grow without producing gas.

Brilliant Green Bile Lactose Broth is formulated for presumptive identification and confirmation of coliform bacteria. Ox-bile and Brilliant green inhibit the growth of gram-positive bacteria including lactose fermenting Clostridia (but not all gram positives). Brilliant Green Bile Lactose Broth is used for the confirmation of presumptive positive tubes showing gas production in Lactose Broth or Lauryl sulfate Broth because of some Gram-positive spore formers may also produce gas in the bile or Brilliant green media.

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