why staning is not requried to observe algea?
Answers
Explanation:
algae usually grows in colonies ....
and impart colour also
hope it helps you mate
Answer:
Explanation:
the usual procedure that I am using is to add 3 volumes of 4% PFA in PBS to your sample, incubate it o/n or at least 3 hours at 4 °C. Wash the sample twice with 1 volume PBS. Resuspend in an appropriate volume of PBS (possibly smaller volumes) before you add another volume of 100% EtOH to reach a 50% EtOH solution to store your sample in. Storing should be done at -20 °C and depending on the sample they might be good for a couple of months, to several years. I think a PFA fixation protocol is also outlined in the paper I mention above.
Cheers!
You can give FISH a go. Bacteria, algae, and fungi can be well separated using fluorescence in situ hybridisation using fluorescence microscopy or flow cytometry. I propose to fix your samples in EtOH but process them asap (on the same day). After reading up on FISH you will understand why. Alternatively you can try and fix the samples using paraformaldehyde, but you might miss gram positive bacteria then. Depending on later questions, you can then use more specific FISH probes to look into specific taxa and phyla. Algae have actually a quite pronounced autofluorescence, so there is no probe needed to pick them up.