Question

Why the leaf is boiled in alcohol over a water bath for iodine test?

Answer 1

The hot water kills the leaf and the alcohol breaks down the chlorophyll, taking the green color out of the leaf. When you put iodine on the leaves, one of them will turn blue-black and the other will be a reddish-brown. Iodine is an indicator that turns blue-black in the presence of starch.

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Answer 2

When leaf is put in hot water bath chlorophyll    dissolves in aq. Alcoholsolution thenWhen you put iodine on the leaves, one of them will turn blue-black and the other will be a reddish-brown. Iodine is an indicator that turns blue-black in the presence of starch.