Why to induce crown gall disesase in tobacco plant?
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The technique of induction and isolation of crown-gall tumours in anther-derived haploid Nicotiana tabacum hosts in sterile culture conditions was developed. The conditions for sterilisation of tumours for their transfer to tissue cultures were found. When following the tumour phenotype stability, characteristic phenovariants with different degrees of morphogenesis were isolated. The possibility of utilizing tumour cell cultures for cytogenetic analysis of the regulative processes of eukaryonts is discussed.
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