Question

Why tris buffer is used for protein gel electrophoresis and tae buffer for nucleic acid gel electrophoresis?

Answer 1

Explanation:

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There are a number of buffers used forelectrophoresis. The most common being, for nucleic acids Tris/Acetate/EDTA (TAE),Tris/Borate/EDTA (TBE). ... Most SDS-PAGE protein separations are performed using a "discontinuous" (or DISC) buffer system that significantly enhances the sharpness of the bands within the gel.

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