Why was a bacterium used in the first instance of the construction of an artificial recombinant dna molecule?
Answers
Answered by
0
There are several reasons why bacteria were chosen to develop recombinant DNA molecules.
Bacteria are simple and readily grow in a matter time. Thus, we can get a number of copies of recombinant DNA within few hours.
Selectable markers are easily available.
They have quite a small genome and less complexity as compared to eukaryotic cells such as yeast and other fungi.
Apart from this, Bacteria have plasmids which are an extra circular genetic material which can be used as a vector (carrier of DNA).
The expression of the genes in the plasmid is independent of the main central circular DNA. Thus, if we ligate any gene of interest in an expression vector, then its expression would occur.
In addition, the genome of the bacteria have unique restriction sites which makes it easy to clone any gene of interest in the specific site.
On the counterpart, eukaryotic gene expression depends on various factors including epigenetic factors, hormones, transcriptional factors and so on. Thus, complex eukaryotic cells cannot be used. They also lack unique restriction sites and the genome of the eukaryotic cell is very large making it very complex.
Bacteria are simple and readily grow in a matter time. Thus, we can get a number of copies of recombinant DNA within few hours.
Selectable markers are easily available.
They have quite a small genome and less complexity as compared to eukaryotic cells such as yeast and other fungi.
Apart from this, Bacteria have plasmids which are an extra circular genetic material which can be used as a vector (carrier of DNA).
The expression of the genes in the plasmid is independent of the main central circular DNA. Thus, if we ligate any gene of interest in an expression vector, then its expression would occur.
In addition, the genome of the bacteria have unique restriction sites which makes it easy to clone any gene of interest in the specific site.
On the counterpart, eukaryotic gene expression depends on various factors including epigenetic factors, hormones, transcriptional factors and so on. Thus, complex eukaryotic cells cannot be used. They also lack unique restriction sites and the genome of the eukaryotic cell is very large making it very complex.
Similar questions