Why we are putting the co spot in thin layer chromatography?
Answers
Answer:
Use of a Co-Spot
TLC can be used to analyze a chemical reaction, for example to determine if the reactants have been consumed and a new product has formed. A pure sample of the reactant can be spotted in one lane of a TLC, and the product mixture in another lane.
Answer:
Thin layer chromatography (TLC) is an extremely valuable technique in the organic lab. It is used to separate mixtures, to check the purity of a mixture, or to monitor the progress of a reaction. The polarity of the solute, polarity of solvent, and polarity of adsorbent are crucial factors that determine the mobility rate of a compound along a TLC plate. This technique helps separate different mixtures of compounds based on their mobility differences. TLC can also be used to identify compounds by comparing it to a known compound
Thin layer chromatography (TLC): this technique was used to separate dried liquids with using liquid solvent (mobile phase) and a glass plate covered with silica gel (stationary phase). Basically, we can use any organic substance (cellulose polyamide, polyethylene, etc.) or inorganic substance (silica gel, aluminum oxide, etc.) in TLC. These substances must be able to divide and form uniform layers. On the surface of the plate, will be a very thin layer of silica which is considered the stationary phase. Then, add a small amount of solvent into a wide-mouth container (i.e. beaker or developing jar) just enough to cover the bottom of the container. Place the prepared TLC plate into the sealed container which has small amount of a solvent (moving phase). Due to capillary action, the solvent moves up to the plate and now we can remove the plate and analyze the Rf values.