Question

Why we collect flowthrough during anion exchange chromatography?

Answer 1

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Ion exchange (IEX) chromatography is a technique that is commonly used in biomolecule purification. It involves the separation of molecules on the basis of their charge.

This technique exploits the interaction between charged molecules in a sample and oppositely charged moieties in the stationery phase of the chromatography matrix. This type of separation is difficult using other techniques as charge is easily manipulated by the pH of buffer used.

Two types of ion exchange separation is possible - cation exchange and anion exchange. In anion exchange the stationary phase is positively charged whilst in cation exchange it is negatively charged.

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