Question

why we do double staining for bignonia

Answer 1

Immunohistochemistry (IHC) is a useful research tool and used to localize specific antigens in tissue sections with labeled antibodies based on antigen-antibody interactions. The immune reactive products can be visualized by a marker including fluorescent dye, enzyme in general; radioactive element or colloidal gold also can be used.

Not only used in research laboratories, IHC is also used in clinical diagnosis; it has been one of the routine diagnostic approaches in daily clinic activities. Prior to the IHC generation, histochemistry was used frequently in clinical diagnosis and laboratory research. However, the histochemistry has been gradually replaced by IHC based on its apparently advantage, the specific antigen-antibody reaction. Theoretically, IHC can identify any existing protein antigen in tissue and thus make it become a critical technique in laboratory research and clinical diagnostic method.

In this article, we aimed to summarize several useful IHC methods that have been used in our laboratories for years. Tissue sampling, preparation, direct staining, indirect staining, double staining etc are described in this article in detail.