Why yeast expression system is better than bacterial expression system?
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Recombinant production of proteins involves transfecting cells with desired gene in a DNA vector. The gene then translates into a protein using host cellular machinery. These expressed proteins can then be extracted by lysing the cells ans subsequent purification steps. Both Prokaryotic and Eukaryotic expression systems are widely used . Each system has its own advantages and disadvantages. A particular expression system is chosen depending on economic and qualitative aspects, such as the type of protein, function and desired protein yield. Bacterial, Yeast, Insect and Mammalian expression systems are widely used. In recent times researchers are also using cell free expression systems.
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