Question

with a neat labelled diagram explain the techniques involved in gene cloning​

Answer 1

Answer:

The basic 7 steps involved in gene cloning are:

Isolation of DNA [gene of interest] fragments to be cloned.

Insertion of isolated DNA into a suitable vector to form recombinant DNA.

Introduction of recombinant DNA into a suitable organism known as host.

Selection of transformed host cells and identification of the clone containing the gene of interest.

Multiplication/Expression of the introduced Gene in the host.

Isolation of multiple gene copies/Protein expressed by the gene.

Purification of the isolated gene copy/protein

Answer 2

$\textbf{Gene Cloning}$ :

1. The carbon copy of an individual is often called a clone. However more appropriately, a clone means to make a genetically exact copy of an organism.

2. In gene cloning, the gene or a piece of DNA fragment is inserted into a bacterial cell where DNA will be multiplied (copied) as the cell divides.

$\textbf{Techniques involved in gene cloning}$ :

$\implies$ Isolation of desired DNA fragment by using restriction enzymes.

$\implies$ Insertion of the DNA fragment into a suitable vector [plasmid] to make rDNA.

$\implies$ Transfer of rDNA into bacterial host cell [Transformation].

$\implies$ Selection and multiplication of recombinant host cell to get a clone.

$\implies$ Expression of cloned gene in host cell.