Write a list of vector used in recombinant dna technology with example and application
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The presence or absence of certain recognition sites in a DNA sample generates variable lengths of DNA fragments, which are separated using gel electrophoresis. They are then hybridized with DNA probes that bind to a complementary DNA sequence in the sample.
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The vectors help in carrying and integrating the desired gene. These form a very important part of the tools of recombinant DNA technology as they are the ultimate vehicles that carry forward the desired gene into the host organism. Plasmids and bacteriophages are the most common vectors in recombinant DNA technology that are used as they have very high copy number. The vectors are made up of an origin of replication- This is a sequence of nucleotide from where the replication starts, a selectable marker – constitute genes which show resistance to certain antibiotics like ampicillin; and cloning sites – the sites recognized by the restriction enzymes where desired DNAs are inserted.
Application of recombinant DNA technology
Application of recombinant DNA technology in Agriculture – For example, manufacture of Bt Cotton to protect the plant against ball worms.
Application of medicines – Insulin production by DNA recombinant technology is a classic example.
Gene Therapy – It is used as an attempt to correct the gene defects which give rise to heredity diseases.
Clinical diagnosis – ELISA is an example where the application of recombinant DNA technology is seen which is used to detect the presence of HIV in a person.
The vectors help in carrying and integrating the desired gene. These form a very important part of the tools of recombinant DNA technology as they are the ultimate vehicles that carry forward the desired gene into the host organism. Plasmids and bacteriophages are the most common vectors in recombinant DNA technology that are used as they have very high copy number. The vectors are made up of an origin of replication- This is a sequence of nucleotide from where the replication starts, a selectable marker – constitute genes which show resistance to certain antibiotics like ampicillin; and cloning sites – the sites recognized by the restriction enzymes where desired DNAs are inserted.
Application of recombinant DNA technology
Application of recombinant DNA technology in Agriculture – For example, manufacture of Bt Cotton to protect the plant against ball worms.
Application of medicines – Insulin production by DNA recombinant technology is a classic example.
Gene Therapy – It is used as an attempt to correct the gene defects which give rise to heredity diseases.
Clinical diagnosis – ELISA is an example where the application of recombinant DNA technology is seen which is used to detect the presence of HIV in a person.
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