Write a note on size our state
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Size exclusion or gel chromatography is a power-full technique that is particularly applicable to high-molecular mass specie, in these technique porous materials as the stationary phase and a liquid as a mobile phase. The diameter of the pore of the porous material is of the order of 50 – 3000Ǻ, which is similar to the size of many molecules. The latter penetrate the pores according to their size. Small molecules penetrate more rapidly than larger molecules, which frequently are excluded from the smaller pores present. This results in a difference in the rates at which the molecules pass down the column, the smaller molecules travelling faster than the larger molecules. In this technique separation takes place according to size of the solute molecule. Exclusion takes place according to molecular size