write a short note on gradient elution
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In gradient elution the eluent strength is increased during the separation by changing the composition of the mobile phase. As a result, the analysis time is reduced and the quality of the separation is improved as well as the detection limit. Binary linear gradients are the most common and the easiest to handle.
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Explanation:
In gradient elution the eluent strength is increased during the separation by changing the composition of the mobile phase. As a result, the analysis time is reduced and the quality of the separation is improved as well as the detection limit. Binary linear gradients are the most common and the easiest to handle.
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