Question

write about the procedure to observe mitochondria?​

Answer 1

Answer:

Mitochondria are found in both plants and animals cells.

It is a 2 walled structure.

The outer mitochondrial membrane is smooth.

The inner mitochondrial is folded into finger like projections. These projections are known as cristae.

The space within mitochondria is known as mitochondrial chamber.

The space filling jell like proteinaceous substance is its matrix.

Mitochondria has its own genetic material.

It is known as semi autonomous organelles.

Explanation:

Mitochondria are shaped perfectly to maximize their productivity. They are made of two membranes. The outer membrane covers the organelle and contains it like a skin. The inner membrane folds over many times and creates layered structures called cristae. The fluid contained in the mitochondria is called the matrix. 

The folding of the inner membrane increases the surface area inside the organelle. Since many of the chemical reactions happen on the inner membrane, the increased surface area creates more space for reactions to occur. If you have more space to work, you can get more work done. Similar surface area strategies are used by microvilli in your intestines. 

What’s in the matrix? It's not like the movies at all. Mitochondria are special because they have their own ribosomes and DNA floating in the matrix. There are also structures called granules which may control concentrations of ions. Cell biologists are still exploring the activity of granules. 

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Answer 2

The procedure to observe mitochondria is as follows:

• Mitochondria is a cell organelle present in both plant and animal cells.

• It is more difficult to stain mitochondria than other organelles because of their small size.

• Special stains like Janus Green are used for the staining.

Procedure:

1. Take onion peel or cheek cells on a slide.
2. Add a drop of water to prevent them from drying.
3. Dilute a few drops of stain like Janus Green-B or MitoView Green with water.
4. Using a dropper, add the diluted stain on the slide having cells.
5. Cover the slide using a coverslip. Avoid any air bubbles.
6. Observe the slide first under the low power of microscope and then under the high power.