write activity about observing cells in human cheek ? with the help of diagram. give me the correct answer
Answers
Answer:
AIM To prepare stained temporary mount of human cheek cells and to record observations and draw labelled diagrams. MATERIALS REQUIRED Slide, coverslip, needle, blotting paper, tooth pick, methylene blue, watch glass, compound microscope. THEORY Animal cells lack cell wall and prominent vacuole. In these cells a semi-permeable membrane called cell membrane surrounds the cytoplasm. In comparison to plant cell, in animal cell cytoplasm is dense and occupies larger space. Vacuoles are absent or very small in size. In animal cells, the position of nucleus is usually central.Cheek cells are part of squamous epithelium. They are thin and flat cells. They found arranged like pavement tiles, i.e., arranged end to end forming a delicate lining.
PROCEDURE
1. Rinse your mouth to remove any food particles.
2. With the help of the toothpick scrap the inner side of the cheek to get some epithelial cells.
3. Place the scrappings in a drop of water over a clean slide.
4. Spread the scrappings with the help of needle.
5. Add a drop of methylene blue over the slide.
6. Wait for 2 minutes and then remove the extra stain with blotting paper.
7. Add a drop of glycerine on the slide.
8. With the help of needle, gently place the coverslip over the slide to avoid entry of air bubbles.
9. Observe the slide under the microscope.
OBSERVATIONS
1. Large number of flat polygonal cells with irregular boundaries attached edge to edge are seen.
2. The cells have thin membrane called cell membrane which encloses jelly like substance .
