Question

write activity on observe the cell in a leaf peel
plzz answer it in proper manner plzzz
like Aim , matériel requrid etcc plzzzzz answer or fast​

Answer 1

Answer:

hi

Explanation:

how are you I hope you are fine mako answers nahi aata ha

Answer 2

Aim

=To prepare a temporary mount of a leaf peel in order to show the stomata of a leaf

Principle/Theory

=Plants are the primary producers. They carry out physiological processes such as photosynthesis and respiration which requires a gas exchange between the tissues of plants and the atmosphere. This process is carried out through tiny openings located in leaves, known as stomata.

Material Required

A potted plant of Bryophyllum or Tradescantia
Needles
Forceps
Watch glass
Dropper
Glass slides
A brush
Coverslips
Blotting paper
Safranin
Compound microscope
Glycerine

Procedure

1. Pick a healthy leaf from the potted plant

2.Fold the leaf to gently pull the peel apart to separate a peeled section from the lower surface of the leaf. Use the forceps to perform this step. Allow the peel to remain in a watch glass holding water for some time.

3.In the watch glass, stain the sample by adding some drops of safranin through a dropper.
Take the peel out after 2-3 minutes. Set it on a clear glass slide

4.Add a drop of glycerin on the peel. Put a clear coverslip over it gently using a needle.

5. Excess glycerin and stain can be removed using blotting paper

6.Examine the slide first under a low-power and then under a high-power magnification of a compound microscope.

Observation

=Visible epidermal cells. The cells in their outline are irregular with no intercellular spaces
=Small openings, stomata are scattered through the epidermal cells
=Guard cells are observed which have chloroplasts and nucleus
=Guard cells are observed having a thin outer covering and a thick inner boundary(concave)
=Guard cells control the closing and opening of the stomata.

Conclusion

=Epidermal cells are found containing stomata on the lower surfaces of the leaf.

Precautions

=Avoid folding the leaf too much. The peel should be snipped to a proper size
=The peel should always be placed at the centre of the slide and the slides should be held from the sides.
=The peel should neither be overstrained nor under strained
=A brush should be used to handle the peel, otherwise would damage cells.
=Glycerin should be used in order to prevent drying of the peel
=Coverslip needs to be placed in such a way that air bubbles are avoided
=Blotting paper can be used to remove excess stain
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